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Western blot analysis of extracts of various cell lines, using EIF3G antibody (19-316) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of U-2 OS cells using EIF3G Polyclonal Antibody (19-316) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using EIF3G antibody (19-316) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

EIF3G Antibody

CATALOG NUMBER: 19-316

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human EIF3G (NP_003746.2).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:200 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
SH-SY5Y
Positive Control 2:
Jurkat
Positive Control 3:
HeLa
Positive Control 4:
NIH/3T3
Positive Control 5:
HL-60
Positive Control 6:
K-562
Predicted Molecular Weight:
Observed: 42kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
EIF3G
Additional Names:
EIF3G, EIF3-P42, EIF3S4, eIF3-delta, eIF3-p44
Ncbi Gene Id Number:
8666
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-316

List Size:
100 uL

List Price:

$436.00

Datasheet
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