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Western blot analysis in K562 cell line lysates (35ug/lane).
EIF3G Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in K562 cell line lysates (35ug/lane).

EIF3G Antibody

CATALOG NUMBER: 61-891

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine, Zebrafish, Mouse, Rat
Immunogen:
This EIF3G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-238 amino acids from the Central region of human EIF3G.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
36 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EIF3G
Additional Names:
Eukaryotic translation initiation factor 3 subunit G {ECO:0000255, HAMAP-Rule:MF_03006}, eIF3g {ECO:0000255, HAMAP-Rule:MF_03006}, Eukaryotic translation initiation factor 3 RNA-binding subunit {ECO:0000255, HAMAP-Rule:MF_03006}, eIF-3 RNA-binding subunit {ECO:0000255, HAMAP-Rule:MF_03006}, Eukaryotic translation initiation factor 3 subunit 4 {ECO:0000255, HAMAP-Rule:MF_03006}, eIF-3-delta {ECO:0000255, HAMAP-Rule:MF_03006}, eIF3 p42 {ECO:0000255, HAMAP-Rule:MF_03006}, eIF3 p44 {ECO:0000255, HAMAP-Rule:MF_03006}, EIF3G {ECO:0000255, HAMAP-Rule:MF_03006}
Protein Accession Number:
O75821
Ncbi Gene Id Number:
8666
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit can bind 18S rRNA.
Background Reference 1:
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18139-18144(2008)
Background Reference 2:
Masutani, M., et al. EMBO J. 26(14):3373-3383(2007)
Background Reference 3:
Damoc, E., et al. Mol. Cell Proteomics 6(7):1135-1146(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-891

List Size:
400 ul

List Price:

$495.00

Datasheet
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