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Western blot analysis of extracts of various cell lines, using EIF3J antibody (23-694) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat brain using EIF3J antibody (23-694) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using EIF3J antibody (23-694) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using EIF3J antibody (23-694) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using EIF3J antibody (23-694) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using EIF3J antibody (23-694) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using EIF3J antibody (23-694) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using EIF3J antibody (23-694) at dilution of 1:100. Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using EIF3J antibody (23-694) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

EIF3J Antibody

CATALOG NUMBER: 23-694

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-258 of human EIF3J (NP_003749.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:200 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
SKOV3
Positive Control 2:
HeLa
Positive Control 3:
Jurkat
Positive Control 4:
A-549
Positive Control 5:
U-87MG
Positive Control 6:
LO2
Predicted Molecular Weight:
Observed: 37kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
EIF3J
Additional Names:
EIF3S1, eIF3-alpha, eIF3-p35, eukaryotic translation initiation factor 3 subunit J, eukaryotic translation initiation factor 3, subunit 1 (alpha, 35kD)
Ncbi Gene Id Number:
8669
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a core subunit of the eukaryotic initiation factor 3 complex, which participates in the initiation of translation by aiding in the recruitment of protein and mRNA components to the 40S ribosome. There are pseudogenes for this gene on chromosomes 1, 3, and 9. Alternative splicing results in multiple transcript variants encoding different isoforms.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-694

List Size:
100 uL

List Price:

$436.00

Datasheet
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