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Western blot analysis in T47D cell line lysates (35ug/lane).
Western blot analysis in T47D cell line lysates (35ug/lane).

EIF3K Antibody

CATALOG NUMBER: 58-722

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This EIF3K antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-204 amino acids from the C-terminal region of human EIF3K.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
25 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EIF3K
Additional Names:
Eukaryotic translation initiation factor 3 subunit K {ECO:0000255, HAMAP-Rule:MF_03010}, eIF3k {ECO:0000255, HAMAP-Rule:MF_03010}, Eukaryotic translation initiation factor 3 subunit 12 {ECO:0000255, HAMAP-Rule:MF_03010}, Muscle-specific gene M9 protein, PLAC-24, eIF-3 p25 {ECO:0000255, HAMAP-Rule:MF_03010}, eIF-3 p28, EIF3K {ECO:0000255, HAMAP-Rule:MF_03010}
Protein Accession Number:
Q9UBQ5
Protein Gi Number:
23396628
Ncbi Gene Id Number:
27335
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The 700-kD eukaryotic translation initiation factor-3(eIF3) is the largest eIF and contains at least 12 subunits,including EIF2S12. eIF3 plays an essential role in translation bybinding directly to the 40S ribosomal subunit and promotingformation of the 40S preinitiation complex (Mayeur et al., 2003[PubMed 14519125]).
Background Reference 1:
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18139-18144(2008)
Background Reference 2:
Lin, Y.M., et al. J. Cell. Sci. 121 (PT 14), 2382-2393 (2008) :
Background Reference 3:
Masutani, M., et al. EMBO J. 26(14):3373-3383(2007)
Background Reference 4:
Damoc, E., et al. Mol. Cell Proteomics 6(7):1135-1146(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-722

List Size:
400 ul

List Price:

$495.00

Datasheet
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