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Western blot analysis of extracts of various cell lines, using EIF4A1 antibody (19-567) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of MCF-7 cells using EIF4A1 antibody (19-567). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of NIH/3T3 cells using 3ug EIF4A1 antibody (19-567). Western blot was performed from the immunoprecipitate using EIF4A1 antibody (19-567) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using EIF4A1 antibody (19-567) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.

EIF4A1 Antibody

CATALOG NUMBER: 19-567

Clonality:
Polyclonal
Tested Applications:
IF, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-406 of human EIF4A1 (NP_001407.1).
Conjugate:
Unconjugated
Tested Applications:
IF, IP, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:20 - 1:50
IP: 1:20 - 1:50
Positive Control 1:
MCF7
Positive Control 2:
22Rv1
Positive Control 3:
HeLa
Positive Control 4:
SW620
Positive Control 5:
Jurkat
Positive Control 6:
NIH/3T3
Predicted Molecular Weight:
Observed: 46kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
EIF4A1
Additional Names:
DDX2A, EIF-4A, EIF4A, eIF-4A-I, eIF4A-I, eukaryotic initiation factor 4A-I, ATP-dependent RNA helicase eIF4A-1, eukaryotic initiation factor 4AI, eukaryotic translation initiation factor 4A
Ncbi Gene Id Number:
1973
User Note:
Optimal dilutions for each application to be determined by the researcher.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-567

List Size:
100 uL

List Price:

$436.00

Datasheet
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