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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Western blot analysis in MCF-7 cell line lysates (35ug/lane).

EIF4A3 Antibody

CATALOG NUMBER: 60-471

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Xenopus, Bovine, Chicken, Zebrafish, Monkey, Mouse, Pig, Rat
Immunogen:
This EIF4A3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-325 amino acids from the C-terminal region of human EIF4A3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EIF4A3
Additional Names:
Eukaryotic initiation factor 4A-III, eIF-4A-III, eIF4A-III, ATP-dependent RNA helicase DDX48, ATP-dependent RNA helicase eIF4A-3, DEAD box protein 48, Eukaryotic initiation factor 4A-like NUK-34, Eukaryotic translation initiation factor 4A isoform 3, Nuclear matrix protein 265, NMP 265, hNMP 265, Eukaryotic initiation factor 4A-III, N-terminally processed, EIF4A3, DDX48, KIAA0111
Protein Accession Number:
P38919
Protein Gi Number:
20532400
Ncbi Gene Id Number:
9775
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the DEAD box protein family.DEAD box proteins, characterized by the conserved motifAsp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They areimplicated in a number of cellular processes involving alterationof RNA secondary structure, such as translation initiation, nuclearand mitochondrial splicing, and ribosome and spliceosome assembly.Based on their distribution patterns, some members of this familyare believed to be involved in embryogenesis, spermatogenesis, andcellular growth and division. The protein encoded by this gene is anuclear matrix protein. Its amino acid sequence is highly similarto the amino acid sequences of the translation initiation factorseIF4AI and eIF4AII, two other members of the DEAD box proteinfamily.
Background Reference 1:
Koroleva, O.A., et al. Plant Cell 21(5):1592-1606(2009)
Background Reference 2:
Karam, R., et al. Oncogene 27(30):4255-4260(2008)
Background Reference 3:
Ma, X.M., et al. Cell 133(2):303-313(2008)
Background Reference 4:
Giorgi, C., et al. Cell 130(1):179-191(2007)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-471

List Size:
400 ul

List Price:

$495.00

Datasheet
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