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Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of anti-EIF4B Antibody Pab in K562 cell line lysates (35ug/lane).
Western blot analysis of anti-EIF4B Antibody Pab in 293 cell line lysates transiently transfected with the EIF4B gene (2ug/lane).
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4B Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.

EIF4B Antibody

CATALOG NUMBER: 60-583

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This EIF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 546-575 amino acids from the C-terminal region of human EIF4B.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
69 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EIF4B
Additional Names:
Eukaryotic translation initiation factor 4B, eIF-4B, EIF4B
Protein Accession Number:
P23588
Protein Gi Number:
205371761
Ncbi Gene Id Number:
1975
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EIF4B is required for the binding of mRNA to ribosomes. It functions in close association with EIF4-F and EIF4-A by binding near the 5'- terminal cap of mRNA in presence of EIF-4F and ATP. This gene also promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F
Background Reference 1:
Doepker, R.C., et al., J. Virol. 78(9):4684-4699 (2004).
Background Reference 2:
Fleming, K., et al., Biochemistry 42(30):8966-8975 (2003).
Background Reference 3:
Milburn, S.C., et al., EMBO J. 9(9):2783-2790 (1990).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-583

List Size:
400 ul

List Price:

$495.00

Datasheet
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