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Western blot analysis of lysed extracts from HT29 cells untreated or treated with Anisomycin using eIF4E (Phospho-Ser209).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF4E (Phospho-Ser209).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed MCF cells using eIF4E (Phospho-Ser209).
Western blot analysis of lysed extracts from HT29 cells untreated or treated with Anisomycin using eIF4E (Phospho-Ser209).

eIF4E (phospho Ser209) Antibody

CATALOG NUMBER: 79-211

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
eIF4E (Phospho-Ser209) antibody was raised against a peptide sequence around phosphorylation site of serine 209 (S-G-S (p) -T-T) derived from Human eIF4E.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of eIF4E only when phosphorylated at serine 209.
Predicted Molecular Weight:
25 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
EIF4E
Additional Names:
CBP, EIF4F, AUTS19, EIF4E1, EIF4EL1, BCL2L3, mRNA cap-binding protein, eIF-4F 25 kDa subunit
Protein Accession Number:
NP_001124150.1
Protein Gi Number:
194578907
Ncbi Gene Id Number:
1977
Background:
Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Background Reference 1:
Li BD, et al. (1998) Ann Surg; 227 (5): 756-763Altmann M, et al. (1989) Nucleic Acids Res; 17 (18): 7520De Gregorio E, et al. (2001) RNA; 7 (1): 106-113Gu W, et al. (2004) Nucleic Acids Res; 32 (15): 4448-4461

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-211

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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