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Antibody used in WB on Human HepG2 at 2.5 ug/ml.
Antibody used in IHC on Human Kidney at 4.0-8.0 ug/ml.
Antibody used in WB on Human HepG2 at 2.5 ug/ml.

EIF4E2 Antibody

CATALOG NUMBER: 29-341

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF4E2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
EIF4E2 antibody can be used for detection of EIF4E2 by ELISA at 1:62500. EIF4E2 antibody can be used for detection of EIF4E2 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
27 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store EIF4E2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
EIF4E2
Additional Names:
EIF4E2, 4EHP, IF4e, 4E-LP, EIF4EL3
Protein Accession Number:
NP_004837
Protein Gi Number:
4757702
Ncbi Gene Id Number:
9470
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EIF4E2 belongs to the eukaryotic initiation factor 4E family. It recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Background Reference 1:
Tee, A.R., FEBS Lett. 564 (1-2), 58-62 (2004).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-341

List Size:
100 ul

List Price:

$473.00

Datasheet
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