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Western blot analysis of EIF4E2 Antibody in NCI-H460 cell line lysates (35ug/lane)
Western blot analysis of EIF4E2 using rabbit polyclonal EIF4E2 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the EIF4E2 gene.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with EIF4E2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of EIF4E2 Antibody in NCI-H460 cell line lysates (35ug/lane)

EIF4E2 Antibody

CATALOG NUMBER: 60-608

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This EIF4E2 antibody is generated from rabbits immunized with human EIF4E2 recombinant protein.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EIF4E2
Additional Names:
Eukaryotic translation initiation factor 4E type 2, eIF-4E type 2, eIF4E type 2, Eukaryotic translation initiation factor 4E homologous protein, Eukaryotic translation initiation factor 4E-like 3, eIF4E-like protein 4E-LP, mRNA cap-binding protein 4EHP, mRNA cap-binding protein type 3, EIF4E2, EIF4EL3
Protein Accession Number:
O60573
Ncbi Gene Id Number:
9470
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EIF4E2 is expressed exclusively in the cytoplasm. This protein recognizes and binds the 7 methylguanosine containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
Background Reference 1:
Rom, E., et al., J. Biol. Chem. 273(21):13104-13109 (1998).
Background Reference 2:
Mao, M., et al., Proc. Natl. Acad. Sci. U.S.A. 95(14):8175-8180 (1998).
Background Reference 3:
Tee, A.R., et al., FEBS Lett. 564 (1-2), 58-62 (2004) (): ().

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-608

List Size:
400 ul

List Price:

$495.00

Datasheet
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