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Antibody used in WB on Human HeLa at 0.2-1 ug/ml.
Antibody used in WB on Human Hela at 1 ug/ml.
Antibody used in WB on Human HeLa at 0.2-1 ug/ml.

EIF5 Antibody

CATALOG NUMBER: 26-794

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human EIF5.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
EIF5 antibody can be used for detection of EIF5 by ELISA at 1:312500. EIF5 antibody can be used for detection of EIF5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1201 - HeLa Cell Lysate
Predicted Molecular Weight:
49 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store EIF5 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
EIF5
Additional Names:
EIF5, EIF-5A, EIF-5
Protein Accession Number:
NP_892116
Protein Gi Number:
37537716
Ncbi Gene Id Number:
1983
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EIF5 catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.mRNA.Met-tRNA[F].eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the release of eIF-2 and the guanine nucleotide. The subsequent joining of a 60S ribosomal subunit results in the formation of a functional 80S initiation complex (80S.mRNA.Met-tRNA[F]).
Background Reference 1:
Olsen, J.V., (2006) Cell 127 (3), 635-648.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

26-794

List Size:
100 ul

List Price:

$519.00

Datasheet
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