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Western blot analysis in CEM cell line lysates (35ug/lane).
EIF5A2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human ovarian carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in CEM cell line lysates (35ug/lane).

EIF5A2 Antibody

CATALOG NUMBER: 57-447

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This EIF5A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-122 amino acids from the C-terminal region of human EIF5A2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
17 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EIF5A2
Additional Names:
Eukaryotic translation initiation factor 5A-2, eIF-5A-2, eIF-5A2, Eukaryotic initiation factor 5A isoform 2, EIF5A2
Protein Accession Number:
Q9GZV4
Protein Gi Number:
74762725
Ncbi Gene Id Number:
56648
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (By similarity).
Background Reference 1:
Tang, D.J., et al. Hepatology 51(4):1255-1263(2010)
Background Reference 2:
Luo, J.H., et al. Cancer Sci. 100(5):896-902(2009)
Background Reference 3:
Chen, W., et al. Cancer Epidemiol. Biomarkers Prev. 18(2):400-408(2009)
Background Reference 4:
Yang, G.F., et al. Gynecol. Oncol. 112(2):314-318(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-447

List Size:
400 ul

List Price:

$495.00

Datasheet
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