Start Your Project

🛍️Explore our Promotions page to discover current deals, limited-time offers, and more!🌟

Western blot analysis of EIG121 in MCF7 cell lysate with EIG121 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of EIG121 in MCF7 cells with EIG121 antibody at 5 μg/mL.
Immunofluorescence of EIG121 in MCF7 cells with EIG121 antibody at 20 μg/mL.
Western blot analysis of EIG121 in MCF7 cell lysate with EIG121 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

EIG121 Antibody

CATALOG NUMBER: 6443

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse: (100%)
Immunogen:
EIG121 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human EIG121.
The immunogen is located within the last 50 amino acids of EIG121.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
EIG121 antibody can be used for detection of EIG121 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
At least four isoforms of EIG121 known to exist. EIG121 antibody is predicted to not cross-react with other UPF0577 protein family members.
Positive Control 1:
Cat. No. 1219 - MCF7 Cell Lysate
Positive Control 2:
Cat. No. 17-012 - MCF7 Cell Slide
Purification:
EIG121 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
EIG121 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
EIG121 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KIAA1324
Additional Names:
EIG121 Antibody: EIG, EIG1
Protein Accession Number:
NP_065826
Protein Gi Number:
38569482
Ncbi Gene Id Number:
57535
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EIG121 Antibody: EIG121 (Estrogen-induced gene 121 protein) is thought to play a role as a marker of hyperestrogenic state and estrogen-related type I endometrial carcinoma. It belongs to the UPF0577 family. It is a 1013 amino acid single-pass transmembrane protein that, though expressed in normal endometrium, is overexpressed in endometriod tumors. As an evolutionarily conserved gene, EIG121 is also expressed during early xenopus development, showing maximum expression at the gastrula stage.
Background Reference 1:
Deng L, Broaddus RR, McCampbell A, et al. Identification of a novel estrogen-regulated gene, EIG121, induced by hormone replacement therapy and differentially expressed in type I and type II endometrial cancer. Clin. Cancer Res. 2005; 11:8258-64.
Background Reference 2:
Deng L, Feng J, Broaddus RR. The novel estrogen-induced gene EIG121 regulates autophagy and promotes cell survival under stress. Cell Death Dis. 2010; 1:e32.
Background Reference 3:
Araki T, Kusakabe M and Nishida E. Expression of estrogen induced gene 121-like (EIG121L) during early Xenopus development. Gene Expr. Patterns 2007; 7:666-71.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6443

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

Customize your order

Bulk Pricing Available

Custom Formulations: azide free, endotoxin testing & endotoxin removal.

Pair your antibody with a Lysate, Peptide, Control, & /OR Secondary Antibody.

Contact our Customer Care Team for more information

Custom Antibody Services

Speak to one of our custom antibody specialists to discuss your next custom antibody project.

New & Featured Products

HIV-1 p24 Antibody [9D2F6] (HRP)
HIV-1 p24 Antibody [9D2F6] (HRP)

CATALOG NUMBER: PM-7369-HRP

HIV-1 p24 Antibody [9D2F6] (biotin)
HIV-1 p24 Antibody [9D2F6] (biotin)

CATALOG NUMBER: PM-7369-biotin

HIV-1 p24 Antibody [9D2F6]
HIV-1 p24 Antibody [9D2F6]

CATALOG NUMBER: PM-7369