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Antibody used in WB on Mouse Trachea at 0.2-1 ug/ml.
Antibody used in WB on Mouse Trachea at 0.2-1 ug/ml.

Elf3 Antibody

CATALOG NUMBER: 29-199

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse Elf3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Elf3 antibody can be used for detection of Elf3 by ELISA at 1:312500. Elf3 antibody can be used for detection of Elf3 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1422 - Mouse Trachea Tissue Lysate
Predicted Molecular Weight:
41 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store Elf3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
Elf3
Additional Names:
Elf3, ESE-1, ESX, jen
Protein Accession Number:
NP_031947
Protein Gi Number:
6679629
Ncbi Gene Id Number:
13710
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Elf3 is a transcriptional activator that binds and transactivates ETS sequences containing the consensus nucleotide core sequence GGA[AT]. It acts synergistically with POU2F3 to transactivate the SPRR2A promoter and with RUNX1 to transactivate the ANGPT1 promoter (By similarity). It also transactivates collagenase, CCL20, CLND7, FLG, KRT8, NOS2, PTGS2, SPRR2B, TGFBR2 and TGM3 promoters. IT represses KRT4 promoter activity (By similarity). It may play an important role in epithelial cell differentiation and tumorigenesis and may be a critical downstream effector of the ERBB2 signaling pathway (By similarity). It may be associated with mammary gland development and involution. It plays an important role in the regulation of transcription with TATA-less promoters in preimplantation embryos, which is essential in preimplantation development.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-199

List Size:
100 ul

List Price:

$519.00

Datasheet
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