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Western blot analysis in HL-60,K562 cell line lysates (35ug/lane).
Flow cytometric analysis of NCI-H292 cells using ELF4 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in HL-60,K562 cell line lysates (35ug/lane).

ELF4 Antibody

CATALOG NUMBER: 62-712

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This ELF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 211-237 amino acids from the Central region of human ELF4.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
71 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ELF4
Additional Names:
ETS-related transcription factor Elf-4, E74-like factor 4, Myeloid Elf-1-like factor, ELF4 {ECO:0000312, EMBL:CAI428821}
Protein Accession Number:
Q99607
Protein Gi Number:
68052244
Ncbi Gene Id Number:
2000
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ELF4 is transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. The protein acts synergistically with RUNX1 to transactivate the IL3 promoter. Also transactivates the PRF1 promoter in natural killer (NK) cells. This protein plays a role in the development and function of NK and NK T-cells and in innate immunity.
Background Reference 1:
Miyazaki Y., Sun X.Oncogene 13:1721-1729(1996)
Background Reference 2:
Aryee D.N.T., Petermann R.Gene 210:71-78(1998)
Background Reference 3:
Mao S., Frank R.C., Zhang J.Mol. Cell. Biol. 19:3635-3644(1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-712

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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