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Western blot of non-phospho- and phospho- recombinant Elk-1 showing specific immunolabeling of the ~46 Elk-1 protein phosphorylated at Ser383.
Western blot of non-phospho- and phospho- recombinant Elk-1 showing specific immunolabeling of the ~46 Elk-1 protein phosphorylated at Ser383.

Elk-1 (phospho Ser383) Antibody

CATALOG NUMBER: XPS-1008

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Elk-1 (Ser383) polyclonal antibody was raised against a synthetic phosphopeptide corresponding to amino acids residues surrounding the phosphorylated Ser383 of human Elk-1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Immunolabeling of the Elk-1 protein band in a Western blot of recombinant Elk-1 is blocked by the phosphopeptide used as antigen but not by the corresponding non-phospho-Elk-1. Applications include Dot Blots (DB) and Western Blots (WB). Suitability for Immunohistochemistry (IHC) has not yet been determined. Human, mouse and rat have 100% amino acid sequence identity with the antigen used to raise the antibody. When internally tested under ideal conditions the working dilutions were 1:1000 for DB and WB.
Specificity:
Elk-1 antibody is specific for ~46k Elk-1 phosphorylated at Ser383 using phospho and dephospho Elk-1 recombinant protein.
Predicted Molecular Weight:
46
Purification:
Affinity Purified
Clonality:
Polyclonal
Physical State:
Liquid
Concentration:
batch dependent
Storage Conditions:
For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.
Ncbi Official Symbol:
ELK1
Protein Accession Number:
P19419
Protein Gi Number:
12643407
Ncbi Gene Id Number:
2002
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Elk-1 is a transcription factor involved in mediating gene transcription in response to growth factors. Elk-1 is thought to be phosphorylated by MAP kinase at Ser383 and phosphorylation at this site is essential for the transcriptional activity of Elk-1. Phosphorylation of Elk-1 has also been implicated in synaptic plasticity in the adult hippocampus.
Background Reference 1:
Li, Q.J., Yang, S.H., Maeda, Y., Sladek, F.M., Sharrocks, A.D., and Martins-Green, M. "MAP kinase phosphorylation-dependent activation of Elk-1 leads to activation of the co-activator p300." EMBO J. 22 (2003), 281 - 91.
Background Reference 2:
Thiels, E., Kanterewicz, B.I., Norman, E.D., Trzaskos, J.M. and Klann, E., "Long-term depression in the adult hippocampus in vivo involves activation of extracellular signal-regulated kinase and phosphorylation of Elk-1," J. Neurosci. 22 (2002) 2054 - 2062.
Background Reference 3:
Hill, C.S. and Treisman, R. "Transcriptional regulation by extracellular signals: mechanisms and specificity," Cell 80 (1995), 199 - 211.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XPS-1008

List Size:
0.1 mL

List Price:

$540.00

Datasheet
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