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Western blot analysis of lysed extracts from 293 and HT29 cells using Elk1 (Ab-389).
<b>Top Image:</b> Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Elk1 (Ab-389).<b>Bottom Image:</b> Immunofluorescence staining of methanol-fixed HeLa cells using Elk1 (Ab-389).
Western blot analysis of lysed extracts from 293 and HT29 cells using Elk1 (Ab-389).

Elk1 (Ab 389) Antibody

CATALOG NUMBER: 79-283

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Elk1 (Ab-389) antibody was raised against a peptide sequence around aa.387~391 (P-R-S-P-A) derived from Human Elk1.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total Elk1 protein.
Predicted Molecular Weight:
62 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
ELK1
Additional Names:
VIL2, ELK1, ETS-domain protein Elk-1
Protein Accession Number:
NP_001107595.1
Protein Gi Number:
166362732
Ncbi Gene Id Number:
2002
Background:
Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. Alternatively spliced transcript variants encoding the same protein have been found for this gene
Background Reference 1:
Janknecht R, et al. (1993) EMBO J. 12 (13): 5097-5104. Marais R, et al. (1993) Cell 73:381-393. Kortenjann M, et al. (1994) Mol Cell Biol. 14:4815-4824. Hill C S, et al. (1995) Cell. 80:199-211. Cavigelli M, et al. (1995) EMBO J. 14:5957-5964.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-283

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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