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Antibody used in WB on Human Jurkat cells at 2.5 ug/ml.
Antibody used in WB on Human Jurkat cells at 2.5 ug/ml.

ELK1 Antibody

CATALOG NUMBER: 28-900

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Dog, Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ELK1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELK1 antibody can be used for detection of ELK1 by ELISA at 1:312500. ELK1 antibody can be used for detection of ELK1 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
45 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ELK1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ELK1
Additional Names:
ELK1,
Protein Accession Number:
NP_005220
Protein Gi Number:
166362730
Ncbi Gene Id Number:
2002
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ELK1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. ELK1 is a nuclear target for the ras-raf-MAPK signaling cascade.This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade.
Background Reference 1:
Hsieh, Y.H., et al., (2006) Biochem. Biophys. Res. Commun. 339 (1), 217-225.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-900

List Size:
100 ul

List Price:

$473.00

Datasheet
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