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Western blot analysis of ELK1 using rabbit polyclonal ELK1 Antibody (S383) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ELK1 gene (Lane 2).
Western blot analysis of ELK1 using rabbit polyclonal ELK1 Antibody (S383) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ELK1 gene (Lane 2).

ELK1 Antibody

CATALOG NUMBER: 62-215

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This ELK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-390 amino acids from human ELK1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
45 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ELK1
Additional Names:
ETS domain-containing protein Elk-1, ELK1
Protein Accession Number:
P19419
Protein Gi Number:
12643407
Ncbi Gene Id Number:
2002
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ELK1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein is a nuclear target for the ras-raf-MAPK signaling cascade.
Background Reference 1:
Salinas, S., et al., J. Cell Biol. 165(6):767-773 (2004).
Background Reference 2:
Yang, S.H., et al., Mol. Cell 12(1):63-74 (2003).
Background Reference 3:
Bebien, M., et al., Oncogene 22(12):1836-1847 (2003).
Background Reference 4:
Sharrocks, A.D., Biochem. Soc. Trans. 30(2):1-9 (2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-215

List Size:
400 ul

List Price:

$495.00

Datasheet
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