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Western blot analysis of Elongin A using rabbit polyclonal Elongin A Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Elongin A gene.
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
Western blot analysis of Elongin A using rabbit polyclonal Elongin A Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Elongin A gene.

Elongin A Antibody

CATALOG NUMBER: 60-375

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
This Elongin A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-127 amino acids from the N-terminal region of human Elongin A.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
90 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
TCEB3
Additional Names:
Transcription elongation factor B polypeptide 3, Elongin 110 kDa subunit, Elongin-A, EloA, RNA polymerase II transcription factor SIII subunit A1, SIII p110, TCEB3
Protein Accession Number:
Q14241
Protein Gi Number:
294862430
Ncbi Gene Id Number:
6924
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Elongin A is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. It activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A functions as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation.
Background Reference 1:
Beausoleil, S.A., et al., Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135 (2004).
Background Reference 2:
Tamura, K., et al., Biochem. Biophys. Res. Commun. 309(1):189-195 (2003).
Background Reference 3:
Kile, B.T., et al., Trends Biochem. Sci. 27(5):235-241 (2002).
Background Reference 4:
Yamazaki, K., et al., J. Biol. Chem. 277(29):26444-26451 (2002).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-375

List Size:
400 ul

List Price:

$495.00

Datasheet
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