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Western blot analysis of extracts of various cell lines, using ELSPBP1 antibody (16-296) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using ELSPBP1 antibody (16-296) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

ELSPBP1 Antibody

CATALOG NUMBER: 16-296

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 110-223 of human ELSPBP1 (NP_071425.3).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
Mouse testis
Positive Control 2:
Rat testis
Predicted Molecular Weight:
Observed: 26kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
ELSPBP1
Additional Names:
E12, EDDM12, EL149, HE12, epididymal sperm-binding protein 1, epididymal protein 12, epididymal secretory protein 12, epididymis luminal secretory protein 149, epididymis secretory sperm binding protein
Ncbi Gene Id Number:
64100
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to the sperm-coating protein family of epididymal origin. This protein and its canine homolog are the first known examples of proteins with four tandemly arranged fibronectin type 2 (Fn2) domains in the Fn2-module protein family.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-296

List Size:
100 uL

List Price:

$436.00

Datasheet
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