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To detect hEMAP-II by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human EMAP-II (XP-5122) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hEMAP-II.
To detect hEMAP-II by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEMAP-II is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hEMAP-II by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEMAP-II is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hEMAP-II by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 – 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Human EMAP-II (XP-5122) as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant hEMAP-II.

EMAP-II Antibody (biotin)

CATALOG NUMBER: XP-5122Bt

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Biotin
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant (human EMAP-II).
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich: To detect hEMAP-II by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Human EMAP-II (XP-5122) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEMAP-II.
Western Blot:
To detect hEMAP-II by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEMAP-II is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hEMAP-II specific antibody was purified by affinity chromatography and then biotinylated.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
EMAP-II antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
AIMP1
Additional Names:
p43, HLD3, EMAP2, SCYE1, EMAPII, Aminoacyl tRNA synthase complex-interacting multifunctional protein 1, Multisynthase complex auxiliary component p43, EMAP-2
Protein Accession Number:
Q12904
Protein Gi Number:
85700432
Ncbi Gene Id Number:
9255
User Note:
Centrifuge vial prior to opening.
Background:
EMAP II is a cytokine that is specifically induced by apoptosis. The release of this cytokine renders the tumor associated vasculature sensitive to tumor necrosis factor. Its precursor is identical to the p43 subunit, which is associated with the multi tRNA synthetase complex. Therefore, proEMAP II may function in binding RNA as part of the tRNA synthetase complex in normal cells and in stimulating inflammatory responses after proteolytic cleavage in tumor cells.EMAP II inhibits angiogenesis of vascular beds and suppresses the growth of primary and secondary tumors without affecting normal tissues.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5122Bt

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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