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Western blot analysis in Hela cell line lysates (35ug/lane).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in Hela cell line lysates (35ug/lane).

EMG1 Antibody

CATALOG NUMBER: 55-653

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This EMG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the Central region of human EMG1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
27 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EMG1
Additional Names:
Ribosomal RNA small subunit methyltransferase NEP1, 211-, 18S rRNA (pseudouridine(1248)-N1)-methyltransferase, 18S rRNA Psi1248 methyltransferase, Nucleolar protein EMG1 homolog, Protein C2f, Ribosome biogenesis protein NEP1, EMG1 {ECO:0000303, PubMed:19463982}
Protein Accession Number:
Q92979
Protein Gi Number:
20532172
Ncbi Gene Id Number:
10436
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes an essential, conserved eukaryoticprotein involved in ribosome biogenesis. In yeast, the relatedprotein is a component of the small subunit processome and isessential for biogenesis of the ribosomal 40S subunit. A mutationin this gene has been associated with Bowen-Conradi syndrome.
Background Reference 1:
Armistead, J., et al. Am. J. Hum. Genet. 84(6):728-739(2009)
Background Reference 2:
Lamesch, P., et al. Genomics 89(3):307-315(2007)
Background Reference 3:
Lamont, R.E., et al. Am. J. Med. Genet.132A (2), 136-143 (2005) :
Background Reference 4:
Bernstein, K.A., et al. Eukaryotic Cell 3(6):1619-1626(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-653

List Size:
400 ul

List Price:

$495.00

Datasheet
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