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45-533 (0.3ug/ml) staining of Mouse Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
45-533 (0.3ug/ml) staining of Mouse Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

EMI2 Antibody

CATALOG NUMBER: 45-533

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Homology:
Expected Species Reactivity based on sequence homology: Human, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-PGSAQSKRNLKRL
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 80kDa band observed in Mouse Ovary lysates (calculated MW of 78.4kDa according to NP_001025031.2). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Human, Rat and Pig Testis, and Human Ovary for this particular batch.
Positive Control 1:
Cat. No. 1210 - 293 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
FBXO43
Additional Names:
FBXO43, EMI2, F-box protein 43, Fbx43, early mitotic inhibitor 2, ERP1
Protein Accession Number:
NP_001025031.2
Protein Gi Number:
117606351
Ncbi Gene Id Number:
286151
Background Reference 1:
Jin J, Cardozo T, Lovering RC, Elledge SJ, Pagano M, Harper JW. Systematic analysis and nomenclature of mammalian F-box proteins. Genes Dev. 2004 Nov 1;18(21):2573-80. No abstract available.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-533

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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