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42-215 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-215 (5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

EML5 Antibody

CATALOG NUMBER: 42-215

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-QQKEPSIDERQG
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Preliminary experiments in Human Brain (Cerebellum, Cerebral Cortex and Hippocampus) lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).Immunohistochemistry:Paraffin embedded Human Small Intestine. Recommended concentration: 5ug/ml.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
EML5
Additional Names:
EML5, echinoderm microtubule associated protein like 5, DKFZp781D1122, EMAP-2, HuEMAP-2
Protein Accession Number:
NP_899243.1
Protein Gi Number:
34485720
Ncbi Gene Id Number:
161436
Background Reference 1:
O'Connor V, Houtman SH, De Zeeuw CI, Bliss TV, French PJ. Eml5, a novel WD40 domain protein expressed in rat brain. Gene 2004 Jul 336 (1): 127-37.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-215

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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