Trial Size

EMX1 Antibody

CATALOG NUMBER: 6625

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Immunogen:
EMX1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human EMX1.
The immunogen is located within amino acids 50 - 100 of EMX1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
EMX1 antibody can be used for detection of EMX1 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in rat samples. All other applications and species not yet tested.
Specificity:
At least two isoforms of EMX1 are known to exist; this antibody will detect the longer isoform. EMX1 antibody is predicted to not cross-react with other EMX family members.
Positive Control 1:
Cat. No. 1464 - Rat Liver Tissue Lysate
Purification:
EMX1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
EMX1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
EMX1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EMX1
Additional Names:
EMX1 Antibody: Homeobox protein EMX1, Empty spiracles homolog 1
Protein Accession Number:
NP_004088
Protein Gi Number:
94536800
Ncbi Gene Id Number:
2016
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EMX1 Antibody: EMX1 is a homeobox transcription factor involved in specifying cell fates in the developing central nervous system and it participates in the development of olfactory neurons. EMX1 is specifically expressed in the developing telencephalic cortex expression, where epxression is restricted to pyramidal neurons. EMX1 is a reliable marker of pyramidal neurons and pyramidal cell lineage. EMX1 has been shown to be one of the downstream target genes for Gli-Kruppel family member 3 (Gli3) transcription factor, which is a part of the Sonic hedgehog-Patched-Gli signaling pathway important in endocrine signaling.
Background Reference 1:
Bishop KM, Garel S, Nakagawa Y, et al. Emx1 and Emx2 cooperate to regulate cortical size, lamination, neuronal differentiation, development of cortical efferents, and thalamocortical pathfinding. J. Comp. Neurol. 2003; 457:345-60.
Background Reference 2:
Lichtneckert R, Nobs L, Reichert H. Empty spiracles is required for the development of olfactory projection neuron circuitry in Drosophila. Development 2008; 135:2415-24
Background Reference 3:
Gulisano M, et al. Emx1 and Emx2 show different patterns of expression during proliferation and differentiation of the developing cerebral cortex in the mouse. Eur. J. Neurosci. 1996; 8:1037-50.
Background Reference 4:
Chan CH, Godinho LN, Thomidou D, et al. 2001. Emx1 is a marker for pyramidal neurons of the cerebral cortex. Cereb. Cortex 11:1191-8.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6625

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

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