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To detect hENA-78 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human ENA-78 (XP-5123Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hENA-78.
To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hENA-78 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.0 ug/ml–2.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
This antibody stained formalin-fixed, paraffin-embedded sections of human colon/rectum adenocarcinoma. The recommended concentration is 1.0 ug/ml–2.0 ug/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
To detect hENA-78 by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci’s Biotinylated Anti-Human ENA-78 (XP-5123Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hENA-78.

ENA-78 Antibody

CATALOG NUMBER: XP-5123

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hENA 78 (human Epithelial Neutrophil Activating Peptide-78).
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:

ELISA:
To detect hENA-78 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hENA-78.
Sandwich: To detect hENA-78 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human ENA-78 (XP-5123Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hENA-78.
Western Blot:
To detect hENA-78 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hENA-78 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-hENA 78 specific antibody was purified by affinity chromatography employing immobilized hENA 78 matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
ENA-78 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
CXCL5
Additional Names:
SCYB5, ENA-78, ENA78, SCYB5, C-X-C motif chemokine 5, Epithelial-derived neutrophil-activating protein 78,
Protein Accession Number:
P42830
Protein Gi Number:
1169525
Ncbi Gene Id Number:
6374
User Note:
Centrifuge vial prior to opening.
Background:
ENA-78 is a a 9.9kD inflammatory chemokine that belongs to the CXC chemokine family. ENA-78 is expressed by fibroblasts, is induced by bacterial lipopolysaccharides, and is chemotactic for neutrophils. This chemokine is produced concomitantly with Interleukin 8 (IL8) in response to stimulation with either Interleukin 1 (IL1) or Tumor Necrosis Factor alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5123

List Size:
0.1 mg

List Price:

$408.00

Datasheet
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