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Western blot analysis in HL-60 cell line lysates (35ug/lane).
ENASE Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in HL-60 cell line lysates (35ug/lane).

ENASE Antibody

CATALOG NUMBER: 55-593

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This ENASE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 326-354 amino acids from the Central region of human ENASE.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
84 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ENGASE
Additional Names:
Cytosolic endo-beta-N-acetylglucosaminidase, ENGase, ENGASE
Protein Accession Number:
Q8NFI3
Protein Gi Number:
74715557
Ncbi Gene Id Number:
64772
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Endo-beta-N-acetylglucosaminidase (ENGase; EC 3.2.1.96) isinvolved in the processing of free oligosaccharides in thecytosol.
Background Reference 1:
Park, K., et al. Diabetes 59(7):1845-1850(2010)
Background Reference 2:
Dorfmueller, H.C., et al. FEBS Lett. 584(4):694-700(2010)
Background Reference 3:
Chantret, I., et al. PLoS ONE(7), E11734 (2010) :
Background Reference 4:
Butkinaree, C., et al. J. Biol. Chem. 283(35):23557-23566(2008)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-593

List Size:
400 ul

List Price:

$495.00

Datasheet
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