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Western blot analysis of ENC-1 in mouse kidney muscle tissue lysate with ENC-1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry of ENC-1 in rat kidney tissue with ENC-1 antibody at 5 μg/mL.
Immunofluorescence of ENC-1 in rat kidney tissue with ENC-1 antibody at 20 μg/mL.
Western blot analysis of ENC-1 in mouse kidney muscle tissue lysate with ENC-1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

ENC-1 Antibody

CATALOG NUMBER: 5693

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human, Mouse, Rat
Immunogen:
ENC-1 antibody was raised against a 13 amino acid synthetic peptide near the center of human ENC-1.
The immunogen is located within amino acids 230 - 280 of ENC-1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
ENC-1 antibody can be used for detection of ENC-1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1405 - Mouse Kidney Tissue Lysate
Purification:
ENC-1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
ENC-1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ENC-1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ENC1
Additional Names:
ENC-1 Antibody: NRPB, CCL28, ENC-1, PIG10, KLHL35, KLHL37, TP53I10, NRPB, Ectoderm-neural cortex protein 1, Kelch-like protein 37
Protein Accession Number:
O14682
Protein Gi Number:
12644050
Ncbi Gene Id Number:
8507
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ENC-1 Antibody: The ectoderm-neural cortex-1 (ENC-1) protein is an early and highly specific marker of neural induction in vertebrates. It is a kelch family related protein that functions as an actin-binding protein and has been suggested to be involved in the organization of the actin cytoskeleton during neural fate specification and development of the nervous system. ENC-1 has also been shown to be required for adipocyte differentiation when cytoskeletal reorganization and cell shape change from fibroblastic preadipocytes to spherical adipocytes occur.
Background Reference 1:
Hernandez MC, Andres-Barquin PJ, Martinez S, et al. ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein. J. Neurosci.1997; 17:3038-51.
Background Reference 2:
Zhao L, Gregoire F and Sul HS. Transient induction of ENC-1, a Kelch-related actin-binding protein, is required for adipocyte differentiation. J. Biol. Chem.2000; 275:16845-50.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5693

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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