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Western blot analysis of ENC-2 in Jurkat cell lysate with ENC-2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Immunocytochemistry of ENC-2 in Jurkat cells with ENC-2 antibody at 5 μg/mL.
Immunofluorescence of ENC-2 in Jurkat cells with ENC-2 antibody at 20 μg/mL.
Western blot analysis of ENC-2 in Jurkat cell lysate with ENC-2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Trial Size

ENC-2 Antibody

CATALOG NUMBER: 5695

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Chicken
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Chicken
Species Reactivity:
Human
Immunogen:
ENC-2 antibody was raised against a 14 amino acid synthetic peptide near the center of human ENC-2.
The immunogen is located within amino acids 230 - 280 of ENC-2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
ENC-2 antibody can be used for detection of ENC-2 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Positive Control 2:
Cat. No. 17-005 - Jurkat Cell Slide
Purification:
ENC-2 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgY
physical-state:
Liquid
Buffer:
ENC-2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
ENC-2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
KLHL25
Additional Names:
ENC-2 Antibody: ENC2, ENC-2, ENC2, Kelch-like protein 25, Ectoderm-neural cortex protein 2
Protein Accession Number:
AAV51405
Protein Gi Number:
55418570
Ncbi Gene Id Number:
64410
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ENC-2 Antibody: The ectoderm-neural cortex-2 (ENC-2) protein a member of the BTB/PDZ Kelch protein family displaying significant homology to ENC-1, an actin-binding protein involved in neural fate specification during embryogenesis and adipocyte maturation. The exact function of ENC-2 has yet to be determined, but it has been suggested to be involved in the differentiation of neuronal precursor cells into neurons and astrocytes.
Background Reference 1:
Hernandez MC, Andres-Barquin PJ, Martinez S, et al. ENC-1: a novel mammalian kelch-related gene specifically expressed in the nervous system encodes an actin-binding protein. J. Neurosci.1997; 17:3038-51.
Background Reference 2:
Zhao L, Gregoire F and Sul HS. Transient induction of ENC-1, a Kelch-related actin-binding protein, is required for adipocyte differentiation. J. Biol. Chem.2000; 275:16845-50.
Background Reference 3:
Ahn JI, Kim SY, Ko MJ, et al. Analysis of gene expression in mouse spinal cord-derived neural precursor cells during neuronal differentiation. Gen. and Infor.2009; 7:85-96.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5695

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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