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43-171 (1ug/ml) staining of Rat Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-171 (5ug/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-171 (1ug/ml) staining of Rat Ovary lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

ENC1 Antibody

CATALOG NUMBER: 43-171

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: TSVSHDKLPKVQC
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 70kDa band observed in Rat Ovary lysates (calculated MW of 66.1kDa according to Rat NP_001003401.1. Recommended concentration: 1-3ug/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse Brain for this particular batch.Immunohistochemistry:Paraffin embedded Human Adrenal Gland. Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize both reported isoforms (NP_003624.1; NP_001243505.1). Reported variants represent identical protein: NP_001243503.1, NP_001243504.1, NP_003624.1.
Predicted Molecular Weight:
Approx 70kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ENC1
Additional Names:
ENC1, ectodermal-neural cortex 1 (with BTB-like domain), CCL28, ENC-1, FLJ39259, KLHL35, KLHL37, NRPB, PIG10, TP53I10, OTTHUMP00000128235, OTTHUMP00000222054, ectoderm-neural cortex protein 1, ectodermal-neural cortex (with BTB-like domain), kelch-like 35, kelch-like 37, kelch-like protein 37, nuclear matrix protein NRP/B, nuclear restricted protein, BTB domain-like (brain), p53-induced gene 10 protein, tumor protein p53 inducible protein 10
Protein Accession Number:
NP_003624.1,NP_001243505.1
Protein Gi Number:
12644050
Ncbi Gene Id Number:
8507
Background Reference 1:
Wang XJ, Zhang DD.Ectodermal-neural cortex 1 down-regulates Nrf2 at the translational level.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-171

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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