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Western blot analysis of ENG using rabbit polyclonal ENG Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ENG gene.
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ENG using rabbit polyclonal ENG Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ENG gene.

ENG Antibody

CATALOG NUMBER: 55-726

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ENG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 265-294 amino acids from the Central region of human ENG.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
71 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ENG
Additional Names:
Endoglin, CD105, ENG, END
Protein Accession Number:
P17813
Protein Gi Number:
3041681
Ncbi Gene Id Number:
2022
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a homodimeric transmembrane proteinwhich is a major glycoprotein of the vascular endothelium. Thisprotein is a component of the transforming growth factor betareceptor complex and it binds TGFB1 and TGFB3 with high affinity.Mutations in this gene cause hereditary hemorrhagic telangiectasia,also known as Osler-Rendu-Weber syndrome 1, an autosomal dominantmultisystemic vascular dysplasia. Alternatively spliced transcriptvariants encoding different isoforms have been found for this gene.
Background Reference 1:
Lopez-Novoa, J.M., et al. Am. J. Physiol. Heart Circ. Physiol. 299 (4), H959-H974 (2010) :
Background Reference 2:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 3:
Zabaneh, D., et al. PLoS ONE(8), E11961 (2010) :
Background Reference 4:
Maynard, S.E., et al. Hypertens Pregnancy 29(3):330-341(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-726

List Size:
400 ul

List Price:

$495.00

Datasheet
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