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Western blot analysis of various lysates using Engrailed 1 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.
Western blot analysis of various lysates using Engrailed 1 Rabbit mAb at 1:1000 dilution. | Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. | Lysates/proteins: 25μg per lane. | Blocking buffer: 3% nonfat dry milk in TBST. | Detection: ECL Basic Kit. | Exposure time: 90s.

Engrailed 1 Rabbit Recombinant Antibody (81-572)

CATALOG NUMBER: 81-572

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse, Rat
Immunogen:
Synthetic peptide. This information is considered to be commercially sensitive.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
WB,1:1000 - 1:2000, ELISA,Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Positive Control 1:
Mouse brain,Rat brain
Predicted Molecular Weight:
40kDa
purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Concentration:
Batch dependent
Storage Conditions:
Store at -20℃. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
EN1
Additional Names:
ENDOVESLB; Engrailed 1
Protein Accession Number:
Q05925
Ncbi Gene Id Number:
2019
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

81-572

List Size:
100 uL

List Price:

$477.00

Datasheet
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