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45-537 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
45-537 (1µg/ml) staining of Mouse Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Engrailed-2 Antibody

CATALOG NUMBER: 45-537

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Human, Dog
Immunogen:
The immunogen for this antibody is: C-NHSTTAKEGKSDSE
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 35kDa band observed in Mouse Brain lysates (calculated MW of f 33.8kDa according to Mouse NP_034264.1). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature. Immunofluorescence: Strong expression of the protein seen in the nuclei of HEK293 cells. Recommended concentration: 10µg/ml.
Positive Control 1:
Cat. No. 1463 - Rat Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
EN2
Additional Names:
Engrailed-2, engrailed homolog 2 , EN2, AUTS1
Protein Accession Number:
NP_001418.2
Protein Gi Number:
7710121
Ncbi Gene Id Number:
2020
Background Reference 1:
Benayed R, Gharani N, Rossman I, Mancuso V, Lazar G, Kamdar S, Bruse SE, Tischfield S, Smith BJ, Zimmerman RA, Dicicco-Bloom E, Brzustowicz LM, Millonig JH. Support for the Homeobox Transcription Factor Gene ENGRAILED 2 as an Autism Spectrum Disorder Susceptibility Locus. Am J Hum Genet. 2005 Nov;77(5):851-68. Epub 2005 Oct 5.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-537

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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