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Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.

ENO1 Antibody

CATALOG NUMBER: 28-068

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ENO1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ENO1 antibody can be used for detection of ENO1 by ELISA at 1:62500. ENO1 antibody can be used for detection of ENO1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
47 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ENO1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ENO1
Additional Names:
ENO1, NNE, PPH, MPB1, ENO1L1
Protein Accession Number:
NP_001419
Protein Gi Number:
4503571
Ncbi Gene Id Number:
2023
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ENO1 is one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.
Background Reference 1:
Ghosh, A.K., et al., (2005) J. Biol. Chem. 280 (14), 14325-14330.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

28-068

List Size:
100 ul

List Price:

$473.00

Datasheet
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