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Antibody used in IHC.
Antibody used in WB on Human Jurkat 0.25 ug/ml.
Antibody used in IHC.

ENO3 Antibody

CATALOG NUMBER: 30-378

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ENO3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ENO3 antibody can be used for detection of ENO3 by ELISA at 1:12500. ENO3 antibody can be used for detection of ENO3 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
47 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store ENO3 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
ENO3
Additional Names:
ENO3, MSE, GSD13
Protein Accession Number:
NP_001967
Protein Gi Number:
301897469
Ncbi Gene Id Number:
2027
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ENO3 is one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in ENO3 gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme.This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in skeletal muscle cells in the adult. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene can be associated with metabolic myopathies that may result from decreased stability of the enzyme. Two transcripts have been identified for this gene that differ only in their 5' UTR.
Background Reference 1:
Li, T.B., (2004) Acta Biochim. Biophys. Sin. (Shanghai) 36 (6), 412-418.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-378

List Size:
100 ul

List Price:

$519.00

Datasheet
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