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Western blot analysis in Jurkat,MCF-7,U251 cell line and mouse brain tissue lysates (35ug/lane).
Flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Confocal immunofluorescent analysis of ENOA Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
ENOA Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in Jurkat,MCF-7,U251 cell line and mouse brain tissue lysates (35ug/lane).

ENOA Antibody

CATALOG NUMBER: 61-572

Clonality:
Polyclonal
Tested Applications:
Flow, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This ENOA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405-434 amino acids from the C-terminal region of human ENOA.
Conjugate:
Unconjugated
Tested Applications:
Flow, IF, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
For IF starting dilution is: 1:10~50
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
47 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ENO1
Additional Names:
Alpha-enolase, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding protein, Enolase 1, MBP-1, MPB-1, Non-neural enolase, NNE, Phosphopyruvate hydratase, Plasminogen-binding protein, ENO1, ENO1L1, MBPB1, MPB1
Protein Accession Number:
P06733
Protein Gi Number:
119339
Ncbi Gene Id Number:
2023
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ENO1 is one of three enolase isoenzymes found in mammals; the protein alpha-enolase, a homodimeric soluble enzyme, and is also a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.
Background Reference 1:
Cappello,P., Int. J. Cancer 125 (3), 639-648 (2009)
Background Reference 2:
Wygrecka,M., Blood 113 (22), 5588-5598 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-572

List Size:
400 ul

List Price:

$495.00

Datasheet
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