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Western blot analysis of lysed extracts from Rat heart, Mouse heart and Rat kidney tissue using eNOS (Ab-1177).
Immunofluorescence staining of methanol-fixed HeLa cells using eNOS (Ab-1177).
Western blot analysis of lysed extracts from Rat heart, Mouse heart and Rat kidney tissue using eNOS (Ab-1177).

eNOS (Ab 1177) Antibody

CATALOG NUMBER: 79-565

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
eNOS (Ab-1177) antibody was raised against a peptide sequence around aa.1175~1179 (T-Q-S-F-S) derived from Human eNOS.
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
Specificity:
This antibody detects endogenous level of total eNOS protein.
Predicted Molecular Weight:
140 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
NOS3
Additional Names:
eNOS, ECNOS, Constitutive NOS, EC-NOS, NOS3, NOSIII
Protein Accession Number:
NP_000594.2
Protein Gi Number:
40254422
Ncbi Gene Id Number:
4846
Background:
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Background Reference 1:
Fulton, D. et al. (1999) Nature 399, 597-601. Harris, M. B. et al. (2001) J. Biol. Chem. 276, 16587-16591. Thomas, S. R. et al. (2002) J. Biol. Chem. 277, 6017-6024.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-565

List Size:
0.1 mg

List Price:

$370.00

Datasheet
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