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Western blot analysis of extracts from Jurkat cells and HepG2 cells using eNOS (Phospho-Thr495) Antibody. The lane on the right is treated with the antigen-specific peptide.
Western blot analysis of extracts from Jurkat cells and HepG2 cells using eNOS (Phospho-Thr495) Antibody. The lane on the right is treated with the antigen-specific peptide.

eNOS (phospho Thr494) Antibody

CATALOG NUMBER: 79-829

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
eNOS (phospho Thr494) antibody was raised against a peptide sequence around phosphorylation site of threonine 494 (R-K-K (p)-T-F derived from Human eNOS .
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
Western Blot: 1:500~1:1000
Specificity:
The antibody detects endogenous levels of eNOS only when phosphorylated at threonine 494.
Predicted Molecular Weight:
140 kDa
Purification:
Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration:
1 mg/mL
Storage Conditions:
Store antibody at -20°C for up to one year.
Ncbi Official Symbol:
NOS3
Additional Names:
cNOS, EC-NOS, ECNOS, NOS, NOS3
Protein Accession Number:
NP_000594.2
Protein Gi Number:
40254422
Ncbi Gene Id Number:
4846
Background:
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
Background Reference 1:
Janssens S.P., J. Biol. Chem. 267:14519-14522(1992).
Background Reference 2:
Janssens S.P., J. Biol. Chem. 267:22694-22694(1992).
Background Reference 3:
Marsden P.A., FEBS Lett. 307:287-293(1992)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

79-829

List Size:
0.1 mg

List Price:

$376.00

Datasheet
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