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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
ENPEP Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

ENPEP Antibody

CATALOG NUMBER: 56-223

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ENPEP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 899-930 amino acids from the C-terminal region of human ENPEP.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
109 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ENPEP
Additional Names:
Glutamyl aminopeptidase, EAP, Aminopeptidase A, AP-A, Differentiation antigen gp160, CD249, ENPEP
Protein Accession Number:
Q07075
Protein Gi Number:
296439445
Ncbi Gene Id Number:
2028
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ENPEP appears to have a role in the catabolic pathway of the renin-angiotensin system. Probably plays a role in regulating growth and differentiation of early B-lineage cells.
Background Reference 1:
Kalsi, G., et al. Hum. Mol. Genet. 19(12):2497-2506(2010)
Background Reference 2:
Rose, J. Phd, et al. Mol. Med. (2010) In press :
Background Reference 3:
Perez, I., et al. Head Neck 31(10):1335-1340(2009)
Background Reference 4:
Sevalle, J., et al. J. Neurochem. 109(1):248-256(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-223

List Size:
400 ul

List Price:

$495.00

Datasheet
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