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43-412 (0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
43-412 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-412 (5ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-412 (0.3ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

ENPP2 / AUTOTAXIN Antibody

CATALOG NUMBER: 43-412

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Immunogen:
The immunogen for this antibody is: PDHLTSCVRPDVRVS
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 100kDa band observed in Human Placenta lysates (calculated MW of 105kDa according to NP_006200.3). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Liver and Brain (Cortex). Recommended concentration: 5ug/ml.
Specificity:
This antibody is expected to recognize iall reported isoforms (NP_006200.3; NP_001035181.1, NP_001124335.1).
Predicted Molecular Weight:
Approx 100kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
ENPP2 / AUTOTAXIN
Additional Names:
ENPP2, ectonucleotide pyrophosphatase/phosphodiesterase 2, ATX, ATX-X, AUTOTAXIN, LysoPLD, NPP2, PD-IALPHA, PDNP2, E-NPP 2, autotaxin-t, ectonucleotide pyrophosphatase/phosphodiesterase family member 2, extracellular lysophospholipase D, phosphodiesterase
Protein Accession Number:
NP_006200.3,NP_001035181.1,NP_001124335.1
Protein Gi Number:
290457674
Ncbi Gene Id Number:
5168
Background Reference 1:
Li S, Xiong C, Zhang J.ATX and LPA receptor 3 are coordinately up-regulated in lipopolysaccharide-stimulated THP-1 cells through PKR and SPK1-mediated pathways.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-412

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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