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Western blot analysis of ENPP7 Antibody in HL-60 cell line lysates (35ug/lane)
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of ENPP7 Antibody in HL-60 cell line lysates (35ug/lane)

ENPP7 Antibody

CATALOG NUMBER: 61-716

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ENPP7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-313 amino acids from the Central region of human ENPP7.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
51 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ENPP7
Additional Names:
Ectonucleotide pyrophosphatase/phosphodiesterase family member 7, E-NPP 7, NPP-7, Alkaline sphingomyelin phosphodiesterase, Intestinal alkaline sphingomyelinase, Alk-SMase, ENPP7 {ECO:0000312, EMBL:AAH414532}
Protein Accession Number:
Q6UWV6
Protein Gi Number:
134047772
Ncbi Gene Id Number:
339221
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ENPP7 converts sphingomyelin to ceramide. ENPP7 also has phospholipase C activity toward palmitoyl lyso-phosphocholine. ENPP7 does not appear to have nucleotide pyrophosphatase activity.
Background Reference 1:
Wu, J., et al. Biochem. J. 386 (PT 1), 153-160 (2005)
Background Reference 2:
Zhang, Z., et al. Protein Sci. 13(10):2819-2824(2004)
Background Reference 3:
Wu, J., et al. Carcinogenesis 25(8):1327-1333(2004)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-716

List Size:
400 ul

List Price:

$495.00

Datasheet
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