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Western Blot at 1:2000 dilution Lane 1: H.heart lysate Lane 2: 293 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: H.brain whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:1000 dilution Lane 1: human heart lysate Lane 2: 293 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: human brain lysate Lane 5: human skeletal muscle lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of ENTPD2 Antibody in K562 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with ENTPD2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot at 1:2000 dilution Lane 1: H.heart lysate Lane 2: 293 whole cell lysate Lane 3: U-87 MG whole cell lysate Lane 4: H.brain whole cell lysate Lysates/proteins at 20 ug per lane.

ENTPD2 Antibody

CATALOG NUMBER: 63-897

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This ENTPD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-113 amino acids from the N-terminal region of human ENTPD2.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:2000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
ENTPD2
Additional Names:
Ectonucleoside triphosphate diphosphohydrolase 2, NTPDase 2, 361-, CD39 antigen-like 1, Ecto-ATP diphosphohydrolase 2, Ecto-ATPDase 2, Ecto-ATPase 2, ENTPD2, CD39L1
Protein Accession Number:
Q9Y5L3
Protein Gi Number:
18203633
Ncbi Gene Id Number:
954
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
ENTPD2 is the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). E-NTPDases are a family of ecto-nucleosidases that hydrolyze 5'-triphosphates. This ecto-ATPase is an integral membrane protein.
Background Reference 1:
Chiang,W.C. Biochemistry 47 (33), 8775-8785 (2008)
Background Reference 2:
Javed,R. Biochemistry 46 (22), 6617-6627 (2007)
Background Reference 3:
Mukasa,T.Biochemistry 44 (33), 11160-11170 (2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-897

List Size:
400 ul

List Price:

$495.00

Datasheet
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