Eotaxin-2 Antibody

CATALOG NUMBER: XP-5125

Clonality:
Polyclonal
Tested Applications:
ELISA, Neutralization Assay, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant murine Eotaxin-2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, Neutralization Assay, WB
Application Note:
Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mEotaxin-2 (100 ng/mL), a concentration of 7.0-8.0 μg/mL of this antibody is required.
ELISA:
To detect murine Eotaxin-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant murine Eotaxin-2.
Sandwich: To detect mEotaxin-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine Eotaxin-2 (XP-5125Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEotaxin-2.
Western Blot:
To detect murine Eotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant murine Eotaxin-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-murine Eotaxin-2 specific antibody was purified by affinity chromatography employing immobilized murine Eotaxin-2 matrix.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Eotaxin-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Ccl24
Additional Names:
CKb-6, MPIF-2, Scya24, C-C motif chemokine 24, Eosinophil chemotactic protein 2,
Protein Accession Number:
Q9JKC0
Protein Gi Number:
13431902
Ncbi Gene Id Number:
56221
User Note:
Centrifuge vial prior to opening.
Background:
Eotaxin is a 74 amino acid, eosinophil chemotactic CC chemokine originally found in bronchoalveolar lavage fluid from allergic inflammatory subjects. It is involved in regulating the recruitment and activation of inflammatory leukocytes, particularly eosinophils. Eotaxin binds and activates the CCR3 chemokine receptor, and may play a fundamental role in the development of allergic responses.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5125

List Size:
0.1 mg

List Price:

$408.00

Shipping Info
Customize your order
Custom Antibody Services

New & Featured Products

CATALOG NUMBER: PM-7369-HRP

CATALOG NUMBER: PM-7369-biotin

CATALOG NUMBER: PM-7369