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Eotaxin-2 Antibody (biotin)

CATALOG NUMBER: XP-5125Bt

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Mouse
Conjugate:
Biotin
Specifications
Host Species:
Goat
Species Reactivity:
Mouse
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mEotaxin-2 (murine Eotaxin-2).
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
ELISA:
Sandwich: To detect mEotaxin-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine Eotaxin-2 (XP-5125) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mEotaxin-2.
Western Blot:
To detect mEotaxin-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEotaxin-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Anti-mEotaxin-2 specific antibody was purified by affinity chromatography and then biotinylated.
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Eotaxin-2 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Ccl24
Additional Names:
CKb-6, MPIF-2, Scya24, C-C motif chemokine 24, Eosinophil chemotactic protein 2,
Protein Accession Number:
Q9JKC0
Protein Gi Number:
13431902
Ncbi Gene Id Number:
56221
User Note:
Centrifuge vial prior to opening.
Background:
Eotaxin is a 74 amino acid, eosinophil chemotactic CC chemokine originally found in bronchoalveolar lavage fluid from allergic inflammatory subjects. It is involved in regulating the recruitment and activation of inflammatory leukocytes, particularly eosinophils. Eotaxin binds and activates the CCR3 chemokine receptor, and may play a fundamental role in the development of allergic responses.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

XP-5125Bt

List Size:
0.05 mg

List Price:

$408.00

Datasheet
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