Eotaxin Antibody [1.3_17H09-02C06]

CATALOG NUMBER: 38-234

Clonality:
Monoclonal
Tested Applications:
ELISA, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Eotaxin antibody is produced in BALB/c mice using highly pure (>98%) recombinant human Eotaxin as the immunizing antigen.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 1.0-2.0 μg/mL of this antibody will detect at least 800 pg/mL of recombinant human Eotaxin when used with our biotinylated antigen affinity purified anti-human Eotaxin as the detection antibody at a concentration of approximately 0.5-1.0 μg/mL.
Western Blot:
To detect hEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEotaxin is 0.5-1.0 ng/lane, under reducing or non-reducing conditions.
Purification:
This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.
Clonality:
Monoclonal
Physical State:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Eotaxin lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
Ncbi Official Symbol:
CCL11
Additional Names:
SCYA11, Eotaxin, C-C motif chemokine 11SCYA11
Protein Accession Number:
P51671
Protein Gi Number:
1706661
Ncbi Gene Id Number:
6356
User Note:
Centrifuge vial prior to opening.
Background:
Eotaxin is a 74 amino acid, eosinophil chemotactic CC chemokine originally found in bronchoalveolar lavage fluid from allergic inflammatory subjects. It is involved in regulating the recruitment and activation of inflammatory leukocytes, particularly eosinophils. Eotaxin binds and activates the CCR3 chemokine receptor, and may play a fundamental role in the development of allergic responses.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

38-234

List Size:
0.5 mg

List Price:

$376.00

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