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Eotaxin Antibody

CATALOG NUMBER: 46-743

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Human
Immunogen:
The immunogen for this antibody is: C-QDSMKYLDQKSPTPK
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:No specific signals and low background in Human Colon, Duodenum, Ileum and Lung lysates - our routinely used western blotting protocol does not allow detection of proteins as small as the ligand derived from its precursor or the orecusor itself with calculated size of 10.7kDa according to NP_002977.1. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any assay results been reported with other antibodies?
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
physical-state:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
CCL11
Additional Names:
CCL11, chemokine (C-C motif) ligand 11, MGC22554, SCYA11, eosinophil chemotactic protein, eotaxin, small inducible cytokine A11, small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
Protein Accession Number:
NP_002977.1
Protein Gi Number:
4506827
Ncbi Gene Id Number:
6356
Background Reference 1:
Issa R, Sorrentino R, Sukkar MB, Sriskandan S, Chung KF, Mitchell JA. Differential regulation of CCL-11/eotaxin-1 and CXCL-8/IL-8 by gram-positive and gram-negative bacteria in human airway smooth muscle cells. Respir Res. 2008 Apr 1;9:30.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-743

List Size:
0.1 mg

List Price:

$391.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.

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