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To detect mEotaxin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine Eotaxin as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mEotaxin.
To detect mEotaxin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine Eotaxin as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mEotaxin.

Eotaxin (CCL11) Antibody (biotin)

CATALOG NUMBER: 38-333

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Mouse
Conjugate:
Biotin
Specifications
Host Species:
Rabbit
Species Reactivity:
Mouse
Immunogen:
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mEotaxin. Anti-Murine Eotaxin specific antibody was purified by affinity chromatography and then biotinylated.
Conjugate:
Biotin
Tested Applications:
ELISA, WB
Application Note:
Sandwich ELISA: To detect mEotaxin by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci’s Polyclonal Anti-Murine Eotaxin as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mEotaxin.
Western Blot
To detect mEotaxin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEotaxin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Purification:
Eotaxin specific antibody was purified by affinity chromatography and then biotinylated
Clonality:
Polyclonal
physical-state:
Lyophilized
Concentration:
batch dependent
Storage Conditions:
Antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Ncbi Official Symbol:
Ccl11
Additional Names:
CCL11
Protein Accession Number:
P48298
Protein Gi Number:
P48298.1
Ncbi Gene Id Number:
20292
User Note:
Centrifuge vial prior to opening.
Background:
Eotaxin is a CC chemokine that signals through the CCR3 receptor. It is produced by IFN-gamma-stimulated endothelial cells and TNF-activated monocytes. Eotaxin selectively chemoattracts eosinophils and, along with Eotaxin-2 and Eotaxin-3, plays a key role in the regulation of eosinophil recruitment in the asthmatic lung and in allergic reactions. Recombinant Murine Eotaxin is an 8.4 kDa protein containing 74 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

38-333

List Size:
0.025 mg

List Price:

$391.00

Datasheet
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