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Western blot analysis in A549 cell line lysates (35ug/lane).
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in A549 cell line lysates (35ug/lane).

EPAS1 Antibody

CATALOG NUMBER: 55-359

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This EPAS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-150 amino acids from the N-terminal region of human EPAS1.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
96 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EPAS1
Additional Names:
Endothelial PAS domain-containing protein 1, EPAS-1, Basic-helix-loop-helix-PAS protein MOP2, Class E basic helix-loop-helix protein 73, bHLHe73, HIF-1-alpha-like factor, HLF, Hypoxia-inducible factor 2-alpha, HIF-2-alpha, HIF2-alpha, Member of PAS protein 2, PAS domain-containing protein 2, EPAS1, BHLHE73, HIF2A, MOP2, PASD2
Protein Accession Number:
Q99814
Protein Gi Number:
32470617
Ncbi Gene Id Number:
2034
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a transcription factor involved in theinduction of genes regulated by oxygen, which is induced as oxygenlevels fall. The encoded protein contains a basic-helix-loop-helixdomain protein dimerization domain as well as a domain found inproteins in signal transduction pathways which respond to oxygenlevels. Mutations in this gene are associated with erythrocytosisfamilial type 4.
Background Reference 1:
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Background Reference 2:
Yi, X., et al. Science 329(5987):75-78(2010)
Background Reference 3:
Bougatef, F., et al. PLoS ONE(8), E12265 (2010) :
Background Reference 4:
Hossein Ghaderian, S.M., et al. Pathology 42(5):446-453(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-359

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

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