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Western blot analysis in 293 cell line lysates (35ug/lane).
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis in 293 cell line lysates (35ug/lane).

EpCAM Antibody

CATALOG NUMBER: 61-933

Clonality:
Polyclonal
Tested Applications:
Flow, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This EpCAM antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-85 amino acids from the N-terminal region of human EpCAM.
Conjugate:
Unconjugated
Tested Applications:
Flow, WB
Application Note:
For WB starting dilution is: 1:1000
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
35 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
EPCAM
Additional Names:
Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, CD326, EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1
Protein Accession Number:
P16422
Protein Gi Number:
160266056
Ncbi Gene Id Number:
4072
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
EpCAM is a carcinoma-associated antigen and is amember of a family that includes at least two type I membraneproteins. This antigen is expressed on most normal epithelial cellsand gastrointestinal carcinomas and functions as a homotypiccalcium-independent cell adhesion molecule. The antigen is beingused as a target for immunotherapy treatment of human carcinomas.
Background Reference 1:
Lu, T.Y., et al. J. Biol. Chem. 285(12):8719-8732(2010)
Background Reference 2:
Raffel, A., et al. Eur. J. Endocrinol. 162(2):391-398(2010)
Background Reference 3:
Mukherjee, S., et al. Am. J. Pathol. 175(6):2277-2287(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-933

List Size:
400 ul

List Price:

$495.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

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